Planted in 1015 A.D., NeoFio grew for five years before receiving life thanks to the Life Sparkle, retrieved from the Hydra Marshes by Serge. Upon awakening, NeoFio immediately displayed intelligence and grasp of the language, thanking Luccia for creating her and deciding to journey with Serge. After Porre seized control of the Manor, NeoFio waited around Hermit's Hideaway when not with Serge directly. She also made good friends with Turnip, who shared her condition of being a sentient plant.
NeoFio's name may be constructed from the particle "neo", which indicates something new, and fiore, the Italian word for "flower". This could be a reference to her status as a created lifeform. It is also interesting to note that fio, in Latin, may mean "I become" or "I am made", both of which are also appropriate to the way she was created.